TY - JOUR AU - Karuppiah, Krishnaveni AU - Murugan, Iniya AU - Sepperumal, Murugesan AU - Ayyanar, Siva PY - 2021/02/17 Y2 - 2024/03/28 TI - A dual responsive probe based on bromo substituted salicylhydrazone moiety for the colorimetric detection of Cd2+ ions and fluorometric detection of F‒ ions: Applications in live cell imaging JF - International Journal of Bioorganic and Medicinal Chemistry JA - 1.0 VL - 1 IS - 1 SE - Articles DO - 10.55124/bmc.v1i1.20 UR - https://ijbc.sciforce.org/IJBC/article/view/20 SP - 1-9 AB - <p>A new fluorimetric and colorimetric dual-mode probe, 4-bromo-2-(hydrazonomethyl) phenol (<strong>BHP</strong>) has been synthesized and successfully utilized for the recognition of Cd<sup>2+</sup>/F<sup>‒</sup> ions in DMSO/H<sub>2</sub>O (9:1, v/v) system. The probe displays dual channel of detection via fluorescence enhancement and colorimetric changes upon binding with F<sup>‒</sup> and Cd<sup>2+</sup> ions respectively. The Job’s plot analysis, ESI-MS studies, Density Functional Theoretical (DFT) calculations, <sup>1</sup>H NMR and <sup>19</sup>F NMR titration results were confirmed and highly supported the 1:1 binding stoichiometry of the probe was complexed with Cd<sup>2+</sup>/F<sup>‒</sup> ions. Furthermore, intracellular detection of F<sup>‒</sup> ions in HeLa cells and fluorescence imaging analysis in Zebrafish embryos results of the probe <strong>BHP</strong> might be used to reveal their potential applications in a biological living system.</p><p> </p> ER -